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Two enzymes of the acetone degradation pathway of Desulfococcus biacutus : coenzyme B<sub>12</sub>‐dependent 2‐hydroxyisobutyryl‐CoA mutase and 3‐hydroxybutyryl‐CoA dehydrogenase

Two enzymes of the acetone degradation pathway of Desulfococcus biacutus : coenzyme B12‐dependent 2‐hydroxyisobutyryl‐CoA mutase and 3‐hydroxybutyryl‐CoA dehydrogenase

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FREY, Jasmin, Fabian SCHNEIDER, Thomas HUHN, Dieter SPITELLER, Bernhard SCHINK, David SCHLEHECK, 2018. Two enzymes of the acetone degradation pathway of Desulfococcus biacutus : coenzyme B12‐dependent 2‐hydroxyisobutyryl‐CoA mutase and 3‐hydroxybutyryl‐CoA dehydrogenase. In: Environmental Microbiology Reports. 10(3), pp. 283-292. eISSN 1758-2229. Available under: doi: 10.1111/1758-2229.12637

@article{Frey2018-06enzym-42563, title={Two enzymes of the acetone degradation pathway of Desulfococcus biacutus : coenzyme B12‐dependent 2‐hydroxyisobutyryl‐CoA mutase and 3‐hydroxybutyryl‐CoA dehydrogenase}, year={2018}, doi={10.1111/1758-2229.12637}, number={3}, volume={10}, journal={Environmental Microbiology Reports}, pages={283--292}, author={Frey, Jasmin and Schneider, Fabian and Huhn, Thomas and Spiteller, Dieter and Schink, Bernhard and Schleheck, David} }

Schneider, Fabian Spiteller, Dieter Huhn, Thomas Schink, Bernhard eng Schleheck, David Frey, Jasmin Huhn, Thomas 2018-06-13T09:30:17Z 2018-06-13T09:30:17Z Degradation of acetone by the sulfate‐reducing bacterium Desulfococcus biacutus involves an acetone‐activation reaction different from that used by aerobic or nitrate‐reducing bacteria, because the small energy budget of sulfate‐reducing bacteria does not allow for major expenditures into ATP‐consuming carboxylation reactions. In the present study, an inducible coenzyme B<sub>12</sub>‐dependent conversion of 2‐hydroxyisobutyryl‐CoA to 3‐hydroxybutyryl‐CoA was demonstrated in cell‐free extracts of acetone‐grown D. biacutus cells, together with a NAD<sup>+</sup>‐dependent oxidation of 3‐hydroxybutyryl‐CoA to acetoacetyl‐CoA. Genes encoding two mutase subunits and a dehydrogenase, which were found previously to be strongly induced during growth with acetone, were heterologously expressed in E. coli. The activities of the purified recombinant proteins matched with the inducible activities observed in cell‐free extracts of acetone‐grown D. biacutus: proteins (IMG locus tags) DebiaDRAFT_04573 and 04574 constituted a B<sub>12</sub>‐dependent 2‐hydroxyisobutyryl‐CoA/3‐hydroxybutyryl‐CoA mutase, and DebiaDRAFT_04571 was a 3‐hydroxybutyryl‐CoA dehydrogenase. Hence, these enzymes play key roles in the degradation of acetone and define an involvement of CoA esters in the pathway. Further, the involvement of 2‐hydroxyisobutyryl‐CoA strongly indicates that the carbonyl‐C<sub>2</sub> of acetone is added, most likely, to formyl‐CoA through a TDP‐dependent enzyme that is co‐induced in acetone‐grown cells and is encoded in the same gene cluster as the identified mutase and dehydrogenase. 2018-06 Schink, Bernhard Schleheck, David Two enzymes of the acetone degradation pathway of Desulfococcus biacutus : coenzyme B<sub>12</sub>‐dependent 2‐hydroxyisobutyryl‐CoA mutase and 3‐hydroxybutyryl‐CoA dehydrogenase Frey, Jasmin Schneider, Fabian Spiteller, Dieter

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