Type of Publication: | Journal article |
URI (citable link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-131201 |
Author: | Deiters, Alexander; Cropp, T. Ashton; Summerer, Daniel; Mukherji, Mridul; Schultz, Peter G. |
Year of publication: | 2004 |
Published in: | Bioorganic & Medicinal Chemistry Letters ; 14 (2004), 23. - pp. 5743-5745. - ISSN 0960-894X |
Pubmed ID: | 15501033 |
DOI (citable link): | https://dx.doi.org/10.1016/j.bmcl.2004.09.059 |
Summary: |
Here, we report a generally applicable PEGylation methodology based on the site-specific incorporation of para-azidophenylalanine into proteins in yeast. The azido group was used in a mild [3+2] cycloaddition reaction with an alkyne derivatized PEG reagent to afford selectively PEGylated protein. This strategy should be useful for the generation of selectively PEGylated proteins for therapeutic applications.
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Subject (DDC): | 540 Chemistry |
Link to License: | In Copyright |
DEITERS, Alexander, T. Ashton CROPP, Daniel SUMMERER, Mridul MUKHERJI, Peter G. SCHULTZ, 2004. Site-specific PEGylation of proteins containing unnatural amino acids. In: Bioorganic & Medicinal Chemistry Letters. 14(23), pp. 5743-5745. ISSN 0960-894X. Available under: doi: 10.1016/j.bmcl.2004.09.059
@article{Deiters2004-12-06Sites-419, title={Site-specific PEGylation of proteins containing unnatural amino acids}, year={2004}, doi={10.1016/j.bmcl.2004.09.059}, number={23}, volume={14}, issn={0960-894X}, journal={Bioorganic & Medicinal Chemistry Letters}, pages={5743--5745}, author={Deiters, Alexander and Cropp, T. Ashton and Summerer, Daniel and Mukherji, Mridul and Schultz, Peter G.} }
Summerer.pdf | 3174 |