Site-specific PEGylation of proteins containing unnatural amino acids

Type of Publication: Journal article
URI (citable link): http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-131201
Author: Deiters, Alexander; Cropp, T. Ashton; Summerer, Daniel; Mukherji, Mridul; Schultz, Peter G.
Year of publication: 2004
Published in: Bioorganic & Medicinal Chemistry Letters ; 14 (2004), 23. - pp. 5743-5745. - ISSN 0960-894X
Pubmed ID: 15501033
DOI (citable link): https://dx.doi.org/10.1016/j.bmcl.2004.09.059
Summary:
Here, we report a generally applicable PEGylation methodology based on the site-specific incorporation of para-azidophenylalanine into proteins in yeast. The azido group was used in a mild [3+2] cycloaddition reaction with an alkyne derivatized PEG reagent to afford selectively PEGylated protein. This strategy should be useful for the generation of selectively PEGylated proteins for therapeutic applications.
Subject (DDC): 540 Chemistry
Link to License: In Copyright

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DEITERS, Alexander, T. Ashton CROPP, Daniel SUMMERER, Mridul MUKHERJI, Peter G. SCHULTZ, 2004. Site-specific PEGylation of proteins containing unnatural amino acids. In: Bioorganic & Medicinal Chemistry Letters. 14(23), pp. 5743-5745. ISSN 0960-894X. Available under: doi: 10.1016/j.bmcl.2004.09.059

@article{Deiters2004-12-06Sites-419, title={Site-specific PEGylation of proteins containing unnatural amino acids}, year={2004}, doi={10.1016/j.bmcl.2004.09.059}, number={23}, volume={14}, issn={0960-894X}, journal={Bioorganic & Medicinal Chemistry Letters}, pages={5743--5745}, author={Deiters, Alexander and Cropp, T. Ashton and Summerer, Daniel and Mukherji, Mridul and Schultz, Peter G.} }

application/pdf Cropp, T. Ashton Schultz, Peter G. Bioorganic & medicinal chemistry letters ; 14 (2004), 23. - S. 5743-5745 terms-of-use Mukherji, Mridul Here, we report a generally applicable PEGylation methodology based on the site-specific incorporation of para-azidophenylalanine into proteins in yeast. The azido group was used in a mild [3+2] cycloaddition reaction with an alkyne derivatized PEG reagent to afford selectively PEGylated protein. This strategy should be useful for the generation of selectively PEGylated proteins for therapeutic applications. Deiters, Alexander Cropp, T. Ashton 2004-12-06 2011-07-01T13:09:46Z Deiters, Alexander Summerer, Daniel eng Site-specific PEGylation of proteins containing unnatural amino acids Mukherji, Mridul Schultz, Peter G. 2011-07-01T13:09:46Z Summerer, Daniel

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