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Stress-induced modulation of NF-κB activation, inflammation-associated gene expression, and cytokine levels in blood of healthy men

Stress-induced modulation of NF-κB activation, inflammation-associated gene expression, and cytokine levels in blood of healthy men

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KUEBLER, Ulrike, Claudia ZUCCARELLA-HACKL, Angela ARPAGAUS, Jutta M. WOLF, Firouzeh FARAHMAND, Roland VON KÄNEL, Ulrike EHLERT, Petra H. WIRTZ, 2015. Stress-induced modulation of NF-κB activation, inflammation-associated gene expression, and cytokine levels in blood of healthy men. In: Brain, Behavior and Immunity. 46, pp. 87-95. ISSN 0889-1591. eISSN 1090-2139

@article{Kuebler2015-05Stres-29657, title={Stress-induced modulation of NF-κB activation, inflammation-associated gene expression, and cytokine levels in blood of healthy men}, year={2015}, doi={10.1016/j.bbi.2014.12.024}, volume={46}, issn={0889-1591}, journal={Brain, Behavior and Immunity}, pages={87--95}, author={Kuebler, Ulrike and Zuccarella-Hackl, Claudia and Arpagaus, Angela and Wolf, Jutta M. and Farahmand, Firouzeh and von Känel, Roland and Ehlert, Ulrike and Wirtz, Petra H.} }

Stress-induced modulation of NF-κB activation, inflammation-associated gene expression, and cytokine levels in blood of healthy men Ehlert, Ulrike eng Zuccarella-Hackl, Claudia Kuebler, Ulrike Wolf, Jutta M. Kuebler, Ulrike 2015-01-30T08:13:43Z von Känel, Roland 2015-01-30T08:13:43Z von Känel, Roland Arpagaus, Angela Arpagaus, Angela Farahmand, Firouzeh Acute psychosocial stress stimulates transient increases in circulating pro-inflammatory plasma cytokines, but little is known about stress effects on anti-inflammatory cytokines or underlying mechanisms. We investigated the stress kinetics and interrelations of pro- and anti-inflammatory measures on the transcriptional and protein level.<br /><br />Forty-five healthy men were randomly assigned to either a stress or control group. While the stress group underwent an acute psychosocial stress task, the second group participated in a non-stress control condition. We repeatedly measured before and up to 120 min after stress DNA binding activity of the pro-inflammatory transcription factor NF-κB (NF-κB-BA) in peripheral blood mononuclear cells, whole-blood mRNA levels of NF-κB, its inhibitor IκBα, and of the pro-inflammatory cytokines interleukin (IL)-1ß and IL-6, and the anti-inflammatory cytokine IL-10. We also repeatedly measured plasma levels of IL-1ß, IL-6, and IL-10.<br /><br />Compared to non-stress, acute stress induced significant and rapid increases in NF-κB-BA and delayed increases in plasma IL-6 and mRNA of IL-1ß, IL-6, and IκBα (p’s < .045). In the stress group, significant increases over time were also observed for NF-κB mRNA and plasma IL-1ß and IL-10 (p’s < .055). NF-κB-BA correlated significantly with mRNA of IL-1β (r = .52, p = .002), NF-κB (r = .48, p = .004), and IκBα (r = .42, p = .013), and marginally with IL-6 mRNA (r = .31, p = .11). Plasma cytokines did not relate to NF-κB-BA or mRNA levels of the respective cytokines.<br /><br />Our data suggest that stress induces increases in NF-κB-BA that relate to subsequent mRNA expression of pro-inflammatory, but not anti-inflammatory cytokines, and of regulatory-cytoplasmic-proteins. The stress-induced increases in plasma cytokines do not seem to derive from de novo synthesis in circulating blood cells. Zuccarella-Hackl, Claudia Wirtz, Petra H. Ehlert, Ulrike Farahmand, Firouzeh Wirtz, Petra H. Wolf, Jutta M. 2015-05

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