Dissecting Ubiquitin Signaling with Linkage-defined and Protease Resistant Ubiquitin Chains
| Type of Publication: | Journal article |
| URI (citable link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-289791 |
| Author: | Schneider, Tatjana; Schneider, Daniel; Rösner, Daniel; Malhotra, Saurav; Mortensen, Franziska; Mayer, Thomas U.; Scheffner, Martin; Marx, Andreas |
| Year of publication: | 2014 |
| Published in: | Angewandte Chemie International Edition ; 53 (2014), 47. - pp. 12925-12929. - ISSN 1433-7851. - eISSN 1521-3773 |
| Pubmed ID: | 25196034 |
| DOI (citable link): | https://dx.doi.org/10.1002/anie.201407192 |
| Summary: |
Ubiquitylation is a complex posttranslational protein modification and deregulation of this pathway has been associated with different human disorders. Ubiquitylation comes in different flavors: Besides mono-ubiquitylation, ubiquitin chains of various topologies are formed on substrate proteins. The fate of ubiquitylated proteins is determined by the linkage-type of the attached ubiquitin chains, however, the underlying mechanism is poorly characterized. Herein, we describe a new method based on codon expansion and click-chemistry-based polymerization to generate linkage-defined ubiquitin chains that are resistant to ubiquitin-specific proteases and adopt native-like functions. The potential of these artificial chains for analyzing ubiquitin signaling is demonstrated by linkage-specific effects on cell-cycle progression.
|
| Subject (DDC): | 540 Chemistry |
| Keywords: | click chemistry, codon expansion, posttranslational modification, ubiquitin chains, unnatural amino acids |
| Link to License: | In Copyright |
| Bibliography of Konstanz: | Yes |
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SCHNEIDER, Tatjana, Daniel SCHNEIDER, Daniel RÖSNER, Saurav MALHOTRA, Franziska MORTENSEN, Thomas U. MAYER, Martin SCHEFFNER, Andreas MARX, 2014. Dissecting Ubiquitin Signaling with Linkage-defined and Protease Resistant Ubiquitin Chains. In: Angewandte Chemie International Edition. 53(47), pp. 12925-12929. ISSN 1433-7851. eISSN 1521-3773. Available under: doi: 10.1002/anie.201407192
@article{Schneider2014-11-17Disse-28979, title={Dissecting Ubiquitin Signaling with Linkage-defined and Protease Resistant Ubiquitin Chains}, year={2014}, doi={10.1002/anie.201407192}, number={47}, volume={53}, issn={1433-7851}, journal={Angewandte Chemie International Edition}, pages={12925--12929}, author={Schneider, Tatjana and Schneider, Daniel and Rösner, Daniel and Malhotra, Saurav and Mortensen, Franziska and Mayer, Thomas U. and Scheffner, Martin and Marx, Andreas} }
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