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On the Structure and Function of the Phytoene Desaturase CRTI from Pantoea ananatis, a Membrane-Peripheral and FAD-Dependent Oxidase/Isomerase

On the Structure and Function of the Phytoene Desaturase CRTI from Pantoea ananatis, a Membrane-Peripheral and FAD-Dependent Oxidase/Isomerase

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SCHAUB, Patrick, Qiuju YU, Sandra GEMMECKER, Pierre POUSSIN-COURMONTAGNE, Justine MAILLIOT, Alastair G. MCEWEN, Sandro GHISLA, Salim AL-BABILI, Jean CAVARELLI, Peter BEYER, 2012. On the Structure and Function of the Phytoene Desaturase CRTI from Pantoea ananatis, a Membrane-Peripheral and FAD-Dependent Oxidase/Isomerase. In: PLoS ONE. 7(6), e39550. eISSN 1932-6203. Available under: doi: 10.1371/journal.pone.0039550

@article{Schaub2012Struc-23681, title={On the Structure and Function of the Phytoene Desaturase CRTI from Pantoea ananatis, a Membrane-Peripheral and FAD-Dependent Oxidase/Isomerase}, year={2012}, doi={10.1371/journal.pone.0039550}, number={6}, volume={7}, journal={PLoS ONE}, author={Schaub, Patrick and Yu, Qiuju and Gemmecker, Sandra and Poussin-Courmontagne, Pierre and Mailliot, Justine and McEwen, Alastair G. and Ghisla, Sandro and Al-Babili, Salim and Cavarelli, Jean and Beyer, Peter}, note={Article Number: e39550} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/23681"> <dc:creator>Mailliot, Justine</dc:creator> <dcterms:abstract xml:lang="eng">CRTI-type phytoene desaturases prevailing in bacteria and fungi can form lycopene directly from phytoene while plants employ two distinct desaturases and two cis-tans isomerases for the same purpose. This property renders CRTI a valuable gene to engineer provitamin A-formation to help combat vitamin A malnutrition, such as with Golden Rice. To understand the biochemical processes involved, recombinant CRTI was produced and obtained in homogeneous form that shows high enzymatic activity with the lipophilic substrate phytoene contained in phosphatidyl-choline (PC) liposome membranes. The first crystal structure of apo-CRTI reveals that CRTI belongs to the flavoprotein superfamily comprising protoporphyrinogen IX oxidoreductase and monoamine oxidase. CRTI is a membrane-peripheral oxidoreductase which utilizes FAD as the sole redox-active cofactor. Oxygen, replaceable by quinones in its absence, is needed as the terminal electron acceptor. FAD, besides its catalytic role also displays a structural function by enabling the formation of enzymatically active CRTI membrane associates. Under anaerobic conditions the enzyme can act as a carotene cis-trans isomerase. In silico-docking experiments yielded information on substrate binding sites, potential catalytic residues and is in favor of single half-site recognition of the symmetrical C 40 hydrocarbon substrate.</dcterms:abstract> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:language>eng</dc:language> <dcterms:title>On the Structure and Function of the Phytoene Desaturase CRTI from Pantoea ananatis, a Membrane-Peripheral and FAD-Dependent Oxidase/Isomerase</dcterms:title> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-06-19T07:47:22Z</dc:date> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/23681/1/Schaub_236818.pdf"/> <dc:contributor>Beyer, Peter</dc:contributor> <dc:contributor>Yu, Qiuju</dc:contributor> <dcterms:bibliographicCitation>PLoS ONE ; 7 (2012), 6. - e39550</dcterms:bibliographicCitation> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:contributor>Mailliot, Justine</dc:contributor> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:creator>Poussin-Courmontagne, Pierre</dc:creator> <dc:contributor>Ghisla, Sandro</dc:contributor> <dc:contributor>Al-Babili, Salim</dc:contributor> <dc:creator>Gemmecker, Sandra</dc:creator> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <dc:creator>Ghisla, Sandro</dc:creator> <dc:contributor>Cavarelli, Jean</dc:contributor> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/23681"/> <dc:creator>Schaub, Patrick</dc:creator> <dc:contributor>Poussin-Courmontagne, Pierre</dc:contributor> <dc:contributor>Schaub, Patrick</dc:contributor> <dc:rights>terms-of-use</dc:rights> <dc:creator>Beyer, Peter</dc:creator> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/23681/1/Schaub_236818.pdf"/> <dc:contributor>McEwen, Alastair G.</dc:contributor> <dc:creator>McEwen, Alastair G.</dc:creator> <dc:creator>Cavarelli, Jean</dc:creator> <dc:creator>Al-Babili, Salim</dc:creator> <dc:creator>Yu, Qiuju</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-06-19T07:47:22Z</dcterms:available> <dcterms:issued>2012</dcterms:issued> <dc:contributor>Gemmecker, Sandra</dc:contributor> </rdf:Description> </rdf:RDF>

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