Using protease inhibitors in antigen presentation assays

Type of Publication: Contribution to a collection
Author: Basler, Michael; Gröttrup, Marcus
Year of publication: 2013
Published in: Antigen Processing / van Endert, Peter (ed.). - Totowa, NJ : Humana Press, 2013. - (Methods in Molecular Biology ; 960). - pp. 31-39. - ISBN 978-1-62703-217-9
Pubmed ID: 23329476
DOI (citable link): https://dx.doi.org/10.1007/978-1-62703-218-6_3
Summary:
The major histocompatibility complex (MHC) class I restricted pathway of antigen processing allows the presentation of intracellular antigens to cytotoxic T lymphocytes. The proteasome is the main protease in the cytoplasm and the nucleus, which is responsible for the generation of most peptide ligands of MHC-I molecules. Peptides produced by the proteasome can be further trimmed or destroyed by numerous cytosolic or endoplasmic reticulum (ER) lumenal proteases. Small molecule inhibitors are useful tools for probing the role of proteases in MHC class I antigen processing. Here, we describe different methods to test the impact of protease inhibitors in antigen presentation assays.
Subject (DDC): 570 Biosciences, Biology
Keywords: Protease, Proteasome, Inhibiton, Antigen presentation, Antigen processing, MHC-I surface staining, lacZ assay, T cell hybridomas, Intracellular cytokine staining, T cell lines
Bibliography of Konstanz: Yes

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BASLER, Michael, Marcus GRÖTTRUP, 2013. Using protease inhibitors in antigen presentation assays. In: VAN ENDERT, Peter, ed.. Antigen Processing. Totowa, NJ:Humana Press, pp. 31-39. ISBN 978-1-62703-217-9. Available under: doi: 10.1007/978-1-62703-218-6_3

@incollection{Basler2013Using-21985, title={Using protease inhibitors in antigen presentation assays}, year={2013}, doi={10.1007/978-1-62703-218-6_3}, number={960}, isbn={978-1-62703-217-9}, address={Totowa, NJ}, publisher={Humana Press}, series={Methods in Molecular Biology}, booktitle={Antigen Processing}, pages={31--39}, editor={van Endert, Peter}, author={Basler, Michael and Gröttrup, Marcus} }

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