Fingerprinting differential active site constraints of ATPases

Cite This

Files in this item

Checksum: MD5:4eb17c1c88f74700742ef39746830bec

HACKER, Stephan M., Norman HARDT, Alexander BUNTRU, Dana PAGLIARINI, Martin MÖCKEL, Thomas U. MAYER, Martin SCHEFFNER, Christof R. HAUCK, Andreas MARX, 2013. Fingerprinting differential active site constraints of ATPases. In: Chemical Science. 4(4), pp. 1588-1596. ISSN 2041-6520. eISSN 2041-6539. Available under: doi: 10.1039/C3SC21916J

@article{Hacker2013Finge-21689, title={Fingerprinting differential active site constraints of ATPases}, year={2013}, doi={10.1039/C3SC21916J}, number={4}, volume={4}, issn={2041-6520}, journal={Chemical Science}, pages={1588--1596}, author={Hacker, Stephan M. and Hardt, Norman and Buntru, Alexander and Pagliarini, Dana and Möckel, Martin and Mayer, Thomas U. and Scheffner, Martin and Hauck, Christof R. and Marx, Andreas} }

<rdf:RDF xmlns:dcterms="" xmlns:dc="" xmlns:rdf="" xmlns:bibo="" xmlns:dspace="" xmlns:foaf="" xmlns:void="" xmlns:xsd="" > <rdf:Description rdf:about=""> <dc:creator>Scheffner, Martin</dc:creator> <dc:date rdf:datatype="">2013-03-08T09:29:02Z</dc:date> <dspace:isPartOfCollection rdf:resource=""/> <dspace:isPartOfCollection rdf:resource=""/> <dcterms:title>Fingerprinting differential active site constraints of ATPases</dcterms:title> <dc:creator>Buntru, Alexander</dc:creator> <dc:creator>Marx, Andreas</dc:creator> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:contributor>Hardt, Norman</dc:contributor> <dcterms:hasPart rdf:resource=""/> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:creator>Möckel, Martin</dc:creator> <bibo:uri rdf:resource=""/> <dcterms:abstract xml:lang="eng">The free energy provided by adenosine triphosphate (ATP) hydrolysis is central to many cellular processes and, therefore, the number of enzymes utilizing ATP as a substrate is almost innumerable. Modified analogues of ATP are a valuable means to understand the biological function of ATPases. Although these enzymes have evolved towards binding to ATP, large differences in active site architectures were found. In order to systematically access the specific active site constraints of different ATPases suitable tools are required. Here, we present the synthesis of six new ATP-based ATPase probes modified at three different positions of the nucleobase and the ribose, respectively. Subsequently, we studied the ATPases focal adhesion kinase FAK, the ubiquitin-activating protein UBA1 and the kinesin Eg5 as examples for ATPases that process ATP by different mechanisms. We find that for each of these enzymes at least one position in ATP can be modified without loss of acceptance by the enzyme. However, the positions at which modifications are tolerated significantly differ between the studied enzymes allowing fingerprints to be drawn for reactivity. The introduced ATP analogues may form the basis for the design of tailored probes with increased affinity and specificity for a specific ATPase of interest.</dcterms:abstract> <dcterms:bibliographicCitation>Chemical Science ; 4 (2013), 4. - S. 1588-1596</dcterms:bibliographicCitation> <dc:creator>Hauck, Christof R.</dc:creator> <dc:contributor>Hauck, Christof R.</dc:contributor> <dc:contributor>Hacker, Stephan M.</dc:contributor> <dc:contributor>Pagliarini, Dana</dc:contributor> <dc:contributor>Möckel, Martin</dc:contributor> <dc:language>eng</dc:language> <dc:creator>Pagliarini, Dana</dc:creator> <dc:contributor>Mayer, Thomas U.</dc:contributor> <dc:contributor>Marx, Andreas</dc:contributor> <dc:creator>Hardt, Norman</dc:creator> <dcterms:isPartOf rdf:resource=""/> <dcterms:available rdf:datatype="">2013-03-08T09:29:02Z</dcterms:available> <dc:contributor>Scheffner, Martin</dc:contributor> <dcterms:rights rdf:resource=""/> <dc:creator>Mayer, Thomas U.</dc:creator> <dc:rights>terms-of-use</dc:rights> <dc:contributor>Buntru, Alexander</dc:contributor> <dcterms:issued>2013</dcterms:issued> <dspace:hasBitstream rdf:resource=""/> <dc:creator>Hacker, Stephan M.</dc:creator> <dcterms:isPartOf rdf:resource=""/> </rdf:Description> </rdf:RDF>

Downloads since Oct 1, 2014 (Information about access statistics)

Mayer216893.pdf 1089

This item appears in the following Collection(s)

Search KOPS


My Account