Type of Publication: | Journal article |
Author: | Zirah, Séverine; Stefanescu, Raluca; Manea, Marilena; Tian, Xiaodan; Cecal, Roxana; Kozin, Sergey A.; Debey, Pascale; Rebuffat, Sylvie; Przybylski, Michael |
Year of publication: | 2004 |
Published in: | Biochemical and Biophysical Research Communications ; 321 (2004), 2. - pp. 324-328. - ISSN 0006-291X |
Pubmed ID: | 15358179 |
DOI (citable link): | https://dx.doi.org/10.1016/j.bbrc.2004.06.150 |
Summary: |
Amyloid plaques associated to Alzheimer’s disease present a high content of zinc ions. We previously showed that the N-terminal region of the amyloid peptide Aβ constitutes an autonomous zinc-binding domain. This region encompasses the previously identified epitope Aβ(4–10) targeted by antibodies capable to reduce amyloid deposition, but the influence of Aβ/Zn binding on the epitope recognition remains unknown. We demonstrate here the effect of Zn2+ ions on the recognition of peptides sharing the sequence of the Aβ N-terminal domain, by two monoclonal antibodies recognizing the β-amyloid(4–10) epitope. The presence of Zn2+, but not of other cations, increased the recognition of the (1–16) peptide, while it was without effect on the recognition of the (1–10) peptide. These findings show a zinc-induced conformational change of the (1–16)-N-terminal region of Aβ, which results in a better accessibility of the Aβ(4–10) epitope to the anti-Aβ antibodies, and suggest a role of zinc in epitope-based vaccination approaches.
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Subject (DDC): | 540 Chemistry |
Keywords: | Alzheimer’s disease, β-Amyloid peptide, Zinc binding, Epitope |
Bibliography of Konstanz: | Yes |
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ZIRAH, Séverine, Raluca STEFANESCU, Marilena MANEA, Xiaodan TIAN, Roxana CECAL, Sergey A. KOZIN, Pascale DEBEY, Sylvie REBUFFAT, Michael PRZYBYLSKI, 2004. Zinc binding agonist effect on the recognition of the β-amyloid (4–10) epitope by anti-β-amyloid antibodies. In: Biochemical and Biophysical Research Communications. 321(2), pp. 324-328. ISSN 0006-291X. Available under: doi: 10.1016/j.bbrc.2004.06.150
@article{Zirah2004-08-20bindi-17609, title={Zinc binding agonist effect on the recognition of the β-amyloid (4–10) epitope by anti-β-amyloid antibodies}, year={2004}, doi={10.1016/j.bbrc.2004.06.150}, number={2}, volume={321}, issn={0006-291X}, journal={Biochemical and Biophysical Research Communications}, pages={324--328}, author={Zirah, Séverine and Stefanescu, Raluca and Manea, Marilena and Tian, Xiaodan and Cecal, Roxana and Kozin, Sergey A. and Debey, Pascale and Rebuffat, Sylvie and Przybylski, Michael} }