Enzymatic synthesis of perfluoroalkylated DNA

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HOLZBERGER, Bastian, Andreas MARX, 2009. Enzymatic synthesis of perfluoroalkylated DNA. In: Bioorganic & Medicinal Chemistry. 17(10), pp. 3653-3658. ISSN 0968-0896. eISSN 1464-3391. Available under: doi: 10.1016/j.bmc.2009.03.063

@article{Holzberger2009Enzym-1103, title={Enzymatic synthesis of perfluoroalkylated DNA}, year={2009}, doi={10.1016/j.bmc.2009.03.063}, number={10}, volume={17}, issn={0968-0896}, journal={Bioorganic & Medicinal Chemistry}, pages={3653--3658}, author={Holzberger, Bastian and Marx, Andreas} }

Holzberger, Bastian 2011-03-22T17:55:15Z Marx, Andreas First publ. in: Bioorganic & Medicinal Chemistry 17 (2009), 10, pp. 3653-3658 Holzberger, Bastian terms-of-use Enzymatic synthesis of perfluoroalkylated DNA 2009 Marx, Andreas eng Thymidine analogues 5-trifluoromethyl-, 5-pentafluoroethyl- and 5-(heptafluoro-n-propyl)-2¡ä-deoxyuridines were synthesised and converted into the corresponding 5¡ä-triphosphates 1a¨Cc. Performing DNA polymerase-catalyzed primer extension reactions these modified nucleotides were incorporated into DNA to create perfluoroalkylated nucleic acids. Although single modified nucleotides were enzymatically incorporated and further elongated quite similar to the natural TTP, the enzymatic synthesis of multi-modified nucleic acids was initial only feasible with modifications at every fourth base. Nevertheless, as the effects of the modified dUTPs on DNA polymerases varied significantly with the used enzyme, Therminator DNA polymerase was proficient in incorporating 11 adjacent 5-trifluoromethyl-2¡ä-deoxyuridine moieties. 2011-03-22T17:55:15Z

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